Auto-Mag® X-Pure Size Select

Auto-Mag® X-Pure Size Select is based on advanced paramagnetic bead technology, enabling highly efficient DNA fragment purification and size selection across a wide range of applications, including NGS library preparation, PCR cleanup, and enzymatic workflows.

Its optimized chemistry selectively binds DNA fragments larger than 100 bp, effectively removing unwanted components such as primers, nucleotides, salts, and enzymes through a streamlined three-step process: binding, washing, and elution.

By adjusting the bead-to-sample ratio, users can precisely control fragment size distribution, making it ideal for demanding NGS workflows that require accurate and reproducible size selection.

Compatible with both manual and automated workflows, Auto-Mag® X-Pure Size Select delivers high-performance DNA purification and size selection—without the premium cost.

Why Choose Auto-Mag® X-Pure Size Select?

• Comparable Performance to Leading SPRI-Based Solutions
  Delivers equivalent DNA recovery, size selection precision, and reproducibility
• Significant Cost Savings
  Reduces per-sample cost without compromising data quality
• High Recovery Efficiency (>100 bp)
  Maximizes yield while effectively removing primers, dimers, nucleotides, and enzymes
• Precise Size Selection Control
  Supports both single- and double-sided selection with tunable fragment cutoffs
• Seamless Workflow Integration
  Fully compatible with standard SPRI protocols and automation platforms
• Consistent, Reproducible Results
  Tight lot-to-lot consistency ensures dependable performance across runs

DNA Cleanup & Size Select Workflow

PCR Cleanrup, Left Side Size Selection

1. Bind PCR amplicon or ds-DNA to magnetic beads.
2. Separate beads from contaminants.
3. Wash magnetic beads with 85% ethanol to remove contaminants.
4. Elute DNA from magnetic beads.
5. Transfer purified DNA to new storage vessels.

 Double Side Size Selection 

1. Confirm the sample volume.
2. Add corresponding volume of Auto-Mag® X-Pure Size Select to the sample for first binding.
3. Separate the beads and transfer supernatant to a new vessels.
4. Add corresponding volume Auto-Mag® X-Pure Size Select to the supernatant for second binding.
5. Separate beads from contaminants.
6. Wash magnetic beads with 85% ethanol to remove contaminants.
7. Elute DNA from magnetic beads.
8. Transfer to new vessels.

Data & References

DNA size selection and Cleanup

Fig 1: DNA size selection was performed using 20 µl of GeneRuler Ultra Low Range DNA Ladder and 100 bp DNA Ladder mix at different ratios of Auto-Mag® X-Pure Size Select NGS volume to DNA sample volume (1.8x to 0.4x). Selected DNA fragments were eluted in 20 µl and analyzed on an Agilent TapeStation 2200.

Comparison of Double-Sided Size Selection

Fig 2. Comparison of electropherograms of  the double-sided size selection on sheared gDNA at 0.7x/0.2x ratio set using Auto-Mag® X-Pure Size Select (NGS) and a similar kit from Company A following the manufacturer’s recommended protocol. DNA was eluted in 25 µL and analyzed on an Agilent TapeStation® 2200.